摘 要 :训练组(男子足球运动员,n等于16)和对照组(男子大学生,n等于16)在功率自行车上完成一次递增负荷力竭运动.运动前、运动后以及运动后24 h采集静脉血,测定外周血淋巴细胞计数、淋巴细胞凋亡率、羟自由基、8—羟基脱氧鸟嘌呤(8—OHdG)和8—氧鸟嘌呤DNA糖基化酶(OGG1).结果发现,运动后即刻,训练组和对照组外周血淋巴细胞计数(P<0.01;P<0.05)、淋巴细胞凋亡率(P<0.05;P<0.01)、羟自由基(P<0.01;P<0.01)、8—OHdG(P<0.01;P<0.01)和OGG1(P<0.01;P<0.01)均高于运动前水平,而训练组OGG1升高幅度高于对照组(P<0.01),其他各指标升高幅度则均明显低于对照组(均为P<0.01).运动后24 h,对照组外周血淋巴细胞计数低于运动前(P<0.01),淋巴细胞凋亡率高于运动前(P<0.01),而训练组则恢复至运动前;两组8—OHdG(P<0.05;P<0.01)均高于运动前,但升高幅度明显低于对照组(P<0.01);OGG1在对照组恢复至运动前,而训练组仍高于运动前水平(P<0.05).因此,长期运动训练通过降低氧化应激水平、提高DNA修复能力、减轻DNA氧化损伤,抑制运动性淋巴细胞凋亡.
关 键 词 :运动训练;外周血淋巴细胞;DNA损伤;细胞凋亡;DNA修复酶
中图分类号:G804.7 文献标识码:A 文章编号:1006—2076(2012)03—0060—06
Abstract:Training group (male football athletes, n等于16) and control group (male normal undergraduate students, n等于16) performed a bout of incremental exhausted exercise test on cycle ergometer. Peripheral blood lymphocyte counts, apoptotic rate of leukocytes, hydroxy radical,8hydroxydeoxyguanosine (8—OHdG) and 8—oxoguanine DNA glycosylase (OGG1) were determined in venous blood before, immediately after and 24 h after exercise test. We found that although lymphocyte count(P<0.01; P<0.05), apoptotic rate(P<0.05; P<0.01), hydroxyl radical(P<0.01;P<0.01), 8—OHdG(P<0.01;P<0.01) and OGG1(P<0.01;P<0.01) increased immediately after exercise test in both groups, the change extent of OGG1 in training group
收稿日期:2012—02—22
基金项目:浙江省教育厅科研计划项目(Y201017123).
作者简介:代志军(1971— ),男,山东潍坊人,硕士,讲师,研究方向篮球教学与训练.
作者单位:1. 潍坊学院,山东 潍坊 261061;2. 温州医学院,浙江 温州 325035
1. Weifang University, Weifang 261061, China;2. Wenzhou Medical College, Whnezhou 325035, China
was significantly higher than that of control group (P<0.01), while that of other indexes were lower than control group (all P<0.01). After 24h, lymphocyte count was lower (P<0.01), apoptotic rate was higher(P<0.01) than that of pre—exercise in control group, which were recovered in training group; 8—OHdG in both group were higher (P<0.05;P<0.01)than pre—exercise but elevation range in training group was lower than control group (P<0.01); OGG1 recovered in control group, while higher than pre—exercise in training group (P<0.05). In conclusion, attenuated oxidative stress, improved DNA repair ability and decreased lymphocyte oxidative DNA damage could be the mechani to prevent exercise induced lymphocytes apoptosis through long—term exercise training.
Key words:exercise training; peripheral blood lymphocyte; DNA damage; apoptosis; DNA repair enzyme
运动可影响免疫系统的机能,其中大强度(剧烈)运动可抑制免疫,表现为运动后外周血淋巴细胞计数降低,上呼吸道感染的发病率升高.研究表明,运动性免疫抑制的发生与运动诱导的淋巴细胞凋亡密切相关,而运动氧化应激造成的DNA损伤是细胞凋亡的主要原因.此外,机体还存在DNA损伤抵御系统,即针对DNA氧化损伤进行有效修复,人体最重要的修复酶是8—氧鸟嘌呤DNA糖基化酶(8—oxoguanine DNA glycosylase,OGG1).有研究显示,运动诱导的淋巴细胞氧化应激和细胞凋亡与受试者的身体机能状态有关,即经过长期训练者(如运动员)其氧化应激和细胞凋亡的程度要明显低于普通人群,但至今其机制未明.本研究旨在观察长期运动训练对一次力竭运动后外周血淋巴细胞凋亡的影响,并探讨氧化应激、DNA损伤、OGG1表达在其间的作用.